Fluorescent staining of intracellular and extracellular bacteria in blood
نویسندگان
چکیده
منابع مشابه
Fluorescent staining of intracellular and extracellular bacteria in blood.
The fluorescent dye ethidium bromide stains Escherichia coli and Staphylococcus aureus in whole blood. The staining is rapid, relatively specific, and does not require fixation of the sample. Furthermore, stained bacteria can be seen microscopically without the need for a final wash to remove unbound dye. By using lysostaphin, an S. aureus-specific lytic enzyme, we have demonstrated that S. aur...
متن کاملSpecific staining of various bacteria with a single fluorescent antiglobulin.
Coons and Kaplan (1950) developed a rapid, sensitive, and specific fluorescent antibody technique for staining viruses in tissue sections. They used a direct staining procedure in which a specific fluorescent antibody preparation is applied directly to the cell being studied. In the indirect procedure (Weller and Coons, 1954), the virus is first reacted with a specific nonfluorescent antiserum ...
متن کاملIntracellular Staining
The principal advantages of simultaneously recording from, and staining of single neurons, buried deep in tissue, is obvious and need not be stressed. Particularly in insects, where most cell somata are electrically silent, there is so far no way to correlate neuronal activity with a particular cell other than to penetrate and to label small fibers simultaneously (Hengstenberg, 1971). Great eff...
متن کاملFluorescent staining of Histoplasma capsulatum.
In an earlier paper from this laboratory (Gordon, 1958) it was demonstrated that fluorescein-labeled specific antiserum employed as a cellular stain will differentiate Candida albicans and Candida tropicalis from other yeasts and yeastlike organisms, including the remaining members of the genus Candida. The present report describes the preparation and use of a similar reagent for detection and ...
متن کاملRapid fluorescent-antibody staining technique.
The rapid fluorescent-antibody staining technique described by Kellogg and Deacon for staining Neisseria gonorrhoeae and Treponema pallidum was applied to fluorescent-antibody tests for group A streptococci and enteropathogenic Escherichia coli. Results obtained with this staining technique were compared with results using the conventional staining procedure; excellent correlation was obtained....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1984
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.19.4.453-456.1984